The Role of Reactive Oxygen Species in Ischaemia-Reperfusion Injury of the Transplanted Kidney

Measurement of reactive oxygen species production and antioxidant system status before and directly after reperfusion of the transplanted kidney and influence of oxidant stress on kidney function in 2 and 6 weeks.

Official Title

The Role of Reactive Oxygen Species in Ischaemia-Reperfusion Injury of the Transplanted Kidney.

Conditions

• Kidney Transplantation

Study Type

Observational

Study Design

Natural History, Cross-Sectional, Defined Population, Prospective Study.

Further Details

Oxidative stress is one of the most important factors in ischaemia-reperfusion injury to the transplanted organ. In kidney transplant recipients following factors were measured: glutathione level, activities of glutathione peroxidase (GSH-PX), catalase (CAT) and superoxide dismutase (SOD) and superoxide anion production by granulocytes in whole blood without and after the stimulation with zymosan.

Blood was sampled from peripheral vein (before reperfusion) and directly from renal vein (5 and 15 minutes after reperfusion).

Transplanted kidney function was evaluated by the incidence and length of Delayed Graft Function and serum creatinine level in 2 and 6 weeks after transplantation.

Study Start

April 2003

Eligibility & Criteria

• Ages Eligible for Study: 18 years and older
• Genders Eligible for Study: Both
• Accepts Healthy Volunteers: No

Inclusion Criteria:

• Consecutive patients accepted for and undergoing cadaveric kidney transplantation

Exclusion Criteria:

• No informed consent

Total Enrolment

60

Contact Details

Zbigniew Wlodarczyk, (MD,PhD)     
Klinika Transplantologii Collegium Medicum UMK w Toruniu 

Location:

Klinika Transplantologii
Szpital Uniwersytecki, ul. M. Sklodowskiej-Curie 9    
Bydgoszcz
Poland, 85-094