Subzonal microinjection ia a micro manipulation technique used for in vitro fertilisation in the late 1980s and early 1990s. The technique involved injecting motile sperm into the shell of an egg, using a microscope. However the technique was inefficient and became redundant with the successful application of intracytoplasmic sperm injection, a technique in which sperm are injected through the egg’s shell, directly into its nucleus.
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